Background: Jaundice is common in the neonatal period, affecting 50.0-60.0% of newborns. Jaundice is not a disease by itself, but rather, a sign that results from hyper-bilirubinemia, the excessive accumulation of bilirubin in the blood, which has toxic effects on the body, such as kernicterus.Aim: was to determine the impact of intervention program for nurses' knowledge and practice regarding neonatal jaundice. Subject and Methods: the research designwas a Quasi-experimental study.  Thesampleconsisted of 51 nurses working in NICU at Mansoura University Children Hospital. Setting: The study was conducted at Neonatal Intensive Care Unit at Mansoura University Children Hospital during the period from August, 2013 to the end of May 2014. Tools:Two tools were used for data collection, an interview questionnaire sheet to assess nurse's general characteristics and knowledge, an observational checklist to assess nurse's practiceResults: revealed that a highly statistically significant differences between pre and post educational program intervention regarding the level of knowledge and practice. Conclusion: the studyconcludedthat the educational program is an effective method to improve nurses' knowledge and practice.Recommendations: The results of the current study recommendedthat, similar training programs should be implemented in similar setting.