Jerusalem artichoke is an untraditional vegetable crop that offers many health benefits to the human body. Therefore, the present study was carried out to estimate the effect of different ratios of Jerusalem artichoke powder on diabetic rats. Male albino rats (25 rats) weighting 160±10 g used in this study. The rats divided into two main groups. The first main group (5 rats) fed on a basal diet (as a negative control group). While the second main group (20 rats) was diabetic induced by a single intraperitoneal injection from alloxan (150 mg/kg b.w.) then divided into four subgroups. One of them fed on a basal diet as the positive control group and the other groups fed on a basal diet containing 10%, 15%, and 20% of Jerusalem artichoke powder for 28 days. The result of sensory evaluation for pan bread fortified with 10% Jerusalem artichoke powder was the best, this level showed non-significant changes, as compared with control pan bread. On the other hand, feeding rats with diets containing Jerusalem artichoke powder led to a marked improvement in all biochemical and biological parameters. The best results have recorded for the group treated with 20% JAT. Additionally, histopathological examination of the pancreas showed that the adding of Jerusalem artichoke tubers to the diets of diabetic rats improved the pancreatic tissue. Therefore, we recommended the use of the Jerusalem artichoke plant in the different food applications