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169725

GROWTH PATTERN OF HYDROCARBON-UTILIZING ISOLATES IN CHEMICALLY DEFINED MEDIUM

Article

Last updated: 22 Jan 2023

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Abstract

Adhesion of five different bacterial strains, isolated from oil-contaminated soil, on suspended droplets of Jet A fuel was different but not absolutely parallel to the observed growth rates of the strains on this fuel. Preconditioning to hydrocarbons as well as direct aeration of the culture medium significantly increased growth rates of the different strains. Jet-A fuel, and motor oil were found to serve as a source of carbon and energy for each of these strains. Conversely, brake fluid did not support growth of any of these strains. The growth of one strain Arthrobacter globiormis (SBI-5) on hydrocarbons predominated over the otther four. Growth of SBI-5 on Jet-A fuel or motor oil was accompanied by production of an emulsifying agent. Growth rate of SBI-5 was monitored by observing optical density (OD546) and total protein in liquid cultures as well as reduction of 2,3,5-triphenyl tetrazolium chloride (TTC) to formazan in liquid and agar cultures. The latter measurements by colorimetry was found to be faster and simpler for screening biodegradation.

DOI

10.21608/zjps.1994.169725

Authors

First Name

Abdel-Halim

Last Name

El-Sayed

MiddleName

-

Affiliation

Department of Microbiology, Faculty of Pharmacy, Zagazig University, Egypt

Email

amel-sayed@pharmacy.zu.edu.eg

City

-

Orcid

-

First Name

Wafaa

Last Name

Mahmoud

MiddleName

-

Affiliation

Department of Microbiology, Faculty of Pharmacy, Zagazig University, Egypt

Email

wmmahmoud@pharmacy.zu.edu.eg

City

-

Orcid

-

First Name

Edward

Last Name

Davis

MiddleName

-

Affiliation

SymBiotech, Inc., Wallingford, CT, USA

Email

edward.davis@yahoo.com

City

-

Orcid

-

First Name

Robert

Last Name

Coughlin

MiddleName

-

Affiliation

SymBiotech, Inc. and Department of Chemical Engineering, University of Connecticut, Storrs, CT, USA

Email

robert.coughlin@yahoo.com

City

-

Orcid

-

Volume

3

Article Issue

2

Related Issue

24774

Issue Date

1994-12-01

Receive Date

1994-09-02

Publish Date

1994-12-01

Page Start

1

Page End

12

Print ISSN

1110-5089

Online ISSN

2356-9786

Link

https://zjps.journals.ekb.eg/article_169725.html

Detail API

https://zjps.journals.ekb.eg/service?article_code=169725

Order

1

Type

Original Article

Type Code

862

Publication Type

Journal

Publication Title

Zagazig Journal of Pharmaceutical Sciences

Publication Link

https://zjps.journals.ekb.eg/

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Article

Created At

22 Jan 2023