From the leaves of Hibiscus cannabinus L., three flavonoidal glycosides were isolated and fully identified using different physical and spectral tools viz; chromatographic techniques (TLC, PC, CC), UV, 1H- and ³C- NMR (1-D and 2-D) experiments, as well as EI- and FAB- mass spectroscopy. These allowed to get confirmation of the structure of these compounds to be kaempferol-7-0-a-L-rhamnoside, apigenin-7-0-B-D-glucoside and kaempferol-3,7 di-O-a-L- rhamnoside. The antibacterial tests of these compounds were also demonstrated.