Gas liquid chromatographic (GLC) analysis of the fatty acids of Iberis gibraltarica , revealed the presence of 15 compounds.11 of which were identified. Plamitic, stearic and oleic acids were the major compounds (88.83%). While in Iberis umbellata, the same study revealed the presence of 16 compounds, 12 of which were identified. Lauric (only identified in I. umbellate), palmitic, stearic and oleic constituted about (74.23%) of the total fatty acid content.From the unsaponifiable matter of I. gibraltarica and after acetylation the 4-demethy! sterol (26, 27-Dinorergosta-7, 22-dien-3B-yl acetate) 1 was isolated. identified on basis of spectroscopical evidence.