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186307

THE POTENTIAL APPLICATIONS OF C-PROTEIN AS IMMUNOREAGENT IN DETECTION, ISOLATION AND QUANTIFICATION OF IgA

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Last updated: 04 Jan 2025

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Abstract

The binding of IgA with C-protein was studied and compared with that of jacalin. SDS- PAGE and Western blotting for various human IgA forms (IgA1, IgA2, serum IgA and secretory IgA) proved the specificity of C-protein binding and the superiority of biotinylated C-protein over biotinylated jacalin as probing immunochemical reagent for IgA detection. The obtained results proved the usefulness of employing C-protein / sepharose columns in isolation of IgA from the whole serum. Although, a linear quantitative relationship was established with pure IgA, the development of ELISA technique for quantification of IgA in whole serum was not possible in the present study. However, a proposed procedure was set for further work.

DOI

10.21608/zjps.1995.186307

Authors

First Name

Kenneth

Last Name

Timmis

MiddleName

-

Affiliation

Gesellschaft Für Biotechnologische Forschung (GBF), Braunschweig, Germany.

Email

kenneth.timmis@yahoo.com

City

-

Orcid

-

First Name

Fathy

Last Name

Serry

MiddleName

-

Affiliation

Department of Microbiology and Immuselogy Facelty of Pharmacy-Zagazig University Zagazig Egypt

Email

fmserry@pharmacy.zu.edu.eg

City

-

Orcid

-

First Name

Hemat

Last Name

Abd El-Latif

MiddleName

-

Affiliation

Department of Microbiology and Immunology Faculty of Pharmacy-Zagazig University Zagazig Egypt

Email

hkabdel-latif@pharmacy.zu.edu.eg

City

-

Orcid

-

First Name

Gursharan

Last Name

Chatwal

MiddleName

-

Affiliation

University of Braunschweig, Germany

Email

gursharan.chatwal@yahoo.com

City

-

Orcid

-

First Name

Ghada

Last Name

Shaker

MiddleName

-

Affiliation

Department of Microbiology, Faculty of Pharmacy, Zagazig University, Egypt

Email

ghshaker@pharmacy.zu.edu.eg

City

-

Orcid

-

Volume

4

Article Issue

1

Related Issue

24771

Issue Date

1995-06-01

Receive Date

1995-01-10

Publish Date

1995-06-01

Page Start

7

Page End

17

Print ISSN

1110-5089

Online ISSN

2356-9786

Article File

ZJPS_Volume 4_Issue 1_Pages 7-17.pdf

PDF . 9.4MB

Link

https://zjps.journals.ekb.eg/article_186307.html

Detail API

https://zjps.journals.ekb.eg/service?article_code=186307

Order

2

Type

Original Article

Type Code

862

Publication Type

Journal

Publication Title

Zagazig Journal of Pharmaceutical Sciences

Publication Link

https://zjps.journals.ekb.eg/

MainTitle

THE POTENTIAL APPLICATIONS OF C-PROTEIN AS IMMUNOREAGENT IN DETECTION, ISOLATION AND QUANTIFICATION OF IgA

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Article

Created At

22 Jan 2023