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185017

EXPRESSION OF MOUSE ANTICREATINE KINASE (MAK 33), FAR FRAGMENT OF IgG ANTIBODY, IN THE METHYLOTROPHIC YEAST HANSENULA POLYMORPHA II. Gamma chain

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Last updated: 04 Jan 2025

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Abstract

The yeast Hapsenula pdymorpha was used as a host for expression of gamma chain peptide of FAb fragment of mouse articreatine kinase-M, IgG antibody. Two expression systems were designed and assembled based on the promoter codon of either of the preferentially highly expressed enzymes, formate dehydrogenase (FMD) and methanol oxidase (MOX) of H. polymorpha. Two expression plasmid vectors, PGAGU and PHAAI, with FMD and MOX promoters, representity, were contsructed and transformed into H. pdymorpha LR9 Expression of gamma chain gene yielded about 400 mg/L and 550 mg/L, respectively 7% and 10% of the total cell protein under the control of FMD and MOX promoters, respectively. The prepro alpha was incompletely processed and most of the gamma chain peptide accumulated within the cells, especially under the control of FMD promoter.

DOI

10.21608/zjps.1996.185017

Authors

First Name

Hassan

Last Name

Abdel-Salam

MiddleName

-

Affiliation

Department of Microbiology, Faculty of Pharmacy, Zagazig University

Email

haabdel-salam@pharmacy.zu.edu.eg

City

-

Orcid

-

First Name

Ralf

Last Name

Gatzke

MiddleName

-

Affiliation

Institut für Mikrobiologie, Heinrich-Heine Universität, Düsseldorf, Universitätsstr. Düsseldorf, Germany.

Email

ralf.gatzke@yahoo.com

City

-

Orcid

-

First Name

Fathy

Last Name

Serry

MiddleName

-

Affiliation

Department of Microbiology and Immuselogy Facelty of Pharmacy-Zagazig University Zagazig Egypt

Email

fmserry@pharmacy.zu.edu.eg

City

-

Orcid

-

First Name

Hemat

Last Name

Abd El-Latif

MiddleName

-

Affiliation

Department of Microbiology and Immunology Faculty of Pharmacy-Zagazig University Zagazig Egypt

Email

hkabdel-latif@pharmacy.zu.edu.eg

City

-

Orcid

-

First Name

Cornelis

Last Name

Hollenberg

MiddleName

-

Affiliation

Institut für Mikrobiologie, Heinrich-Heine Universität, Düsseldorf, Universitätsstr. Düsseldorf, Germany.

Email

cornelis.hollenberg@yahoo.com

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-

Orcid

-

Volume

5

Article Issue

2

Related Issue

24770

Issue Date

1996-12-01

Receive Date

1996-08-29

Publish Date

1996-12-01

Page Start

62

Page End

70

Print ISSN

1110-5089

Online ISSN

2356-9786

Article File

ZJPS_Volume 5_Issue 2_Pages 62-70.pdf

PDF . 5.9MB

Link

https://zjps.journals.ekb.eg/article_185017.html

Detail API

https://zjps.journals.ekb.eg/service?article_code=185017

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Original Article

Type Code

862

Publication Type

Journal

Publication Title

Zagazig Journal of Pharmaceutical Sciences

Publication Link

https://zjps.journals.ekb.eg/

MainTitle

EXPRESSION OF MOUSE ANTICREATINE KINASE (MAK 33), FAR FRAGMENT OF IgG ANTIBODY, IN THE METHYLOTROPHIC YEAST HANSENULA POLYMORPHA II. Gamma chain

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Article

Created At

22 Jan 2023