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184799

PURIFICATION AND CHARACTERISTICS OF POLYHYDROXY ALKANOATE DEPOLYMERASES FROM EIKENELLA CORRODENS , KLEBSIELLA PNEUMONAE ANDALCALIGENES FAECALIS

Article

Last updated: 22 Jan 2023

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Abstract

In a previous screening study for polyhydroxy alkanoate (PHA) degrading activities, three isolates were obtained and characterized as Eikenella corrondens, Klebsiella pneumoniae and Alcaligenes faecalis . In the present study, PHA depolymerases from these three isolates were purified by DEAE anion exchange chromatography and by chromatofocusing ( isoelectric focusing ) after precipitation from the crude enzyme preparations by ammonium sulfate. The molecular weights for the three enzymes were determined by gel permeation sodium dodecyl sulphat (SDS) polyacrylamide gel eclectrophoresis.

DOI

10.21608/zjps.1998.184799

Authors

First Name

Nehal

Last Name

Yousef

MiddleName

-

Affiliation

Department of Microbiology, Faculty of Pharmacy, University of Zagazig - Egypt

Email

neyousef@pharmacy.zu.edu.eg

City

-

Orcid

-

First Name

Hemat

Last Name

Abd El-Latif

MiddleName

-

Affiliation

Department of Microbiology and Immunology Faculty of Pharmacy-Zagazig University Zagazig Egypt

Email

hkabdel-latif@pharmacy.zu.edu.eg

City

-

Orcid

-

First Name

Fathy

Last Name

Serry

MiddleName

-

Affiliation

Department of Microbiology and Immuselogy Facelty of Pharmacy-Zagazig University Zagazig Egypt

Email

fmserry@pharmacy.zu.edu.eg

City

-

Orcid

-

First Name

J. A.

Last Name

Cameron

MiddleName

-

Affiliation

Department of Microbiology, Molecular and Cell Biology, Connecticut University , Storrs, U.S.A

Email

j.cameron@yahoo.com

City

-

Orcid

-

Volume

7

Article Issue

2

Related Issue

24766

Issue Date

1998-12-01

Receive Date

1998-09-19

Publish Date

1998-12-01

Page Start

46

Page End

54

Print ISSN

1110-5089

Online ISSN

2356-9786

Article File

ZJPS_Volume 7_Issue 2_Pages 46-54.pdf

PDF . 2.5MB

Link

https://zjps.journals.ekb.eg/article_184799.html

Detail API

https://zjps.journals.ekb.eg/service?article_code=184799

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6

Type

Original Article

Type Code

862

Publication Type

Journal

Publication Title

Zagazig Journal of Pharmaceutical Sciences

Publication Link

https://zjps.journals.ekb.eg/

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Article

Created At

22 Jan 2023