184798

MICROBIAL DEGRADATION OF THE MICROBIAL POLYESTER CO-POLYMER POLY HYDROXY BUTYRATE/VALERATE

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Last updated: 04 Jan 2025

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Abstract

Screening of environmental specimens was performed in order to isolate polyhydroxy - butyrate / valerate degrading bacteria. Basal mineral salts medium supplemented with the polymer was used as enrichment medium. Isolates capable of degrading the polymer and hence clearing the opacity of the polymer containing solid medium were selected. The isolates capable of degrading the polyme were obtained and characterized a Ekinella corrodenes , Alcaligenes faecalis and Klebsiella pneumoniae. The three  isolates were found to show extracellular polyhydroxy alkanoate ( PHA) depolymerase activity. Crude enzyme preparations were used to study the characteristics of these enzymes. The optimum temperatures and pHs for activity, substrate specificity, effect of dialysis , divalent cations, various inhibitors and detergents on depolymeterase and esterase activities were studied for the three isolates. The effect of cultural and nutritional conditions on enzymes production were studied for E. corrodenes .

DOI

10.21608/zjps.1998.184798

Authors

First Name

Nehal

Last Name

Yousef

MiddleName

-

Affiliation

Department of Microbiology, Faculty of Pharmacy, University of Zagazig - Egypt

Email

neyousef@pharmacy.zu.edu.eg

City

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Orcid

-

First Name

Hemat

Last Name

Abd El-Latif

MiddleName

-

Affiliation

Department of Microbiology and Immunology Faculty of Pharmacy-Zagazig University Zagazig Egypt

Email

hkabdel-latif@pharmacy.zu.edu.eg

City

-

Orcid

-

First Name

Fathy

Last Name

Serry

MiddleName

-

Affiliation

Department of Microbiology and Immuselogy Facelty of Pharmacy-Zagazig University Zagazig Egypt

Email

fmserry@pharmacy.zu.edu.eg

City

-

Orcid

-

First Name

J. A.

Last Name

Cameron

MiddleName

-

Affiliation

Department of Microbiology, Molecular and Cell Biology, Connecticut University , Storrs, U.S.A

Email

j.cameron@yahoo.com

City

-

Orcid

-

Volume

7

Article Issue

2

Related Issue

24766

Issue Date

1998-12-01

Receive Date

1998-09-10

Publish Date

1998-12-01

Page Start

33

Page End

45

Print ISSN

1110-5089

Online ISSN

2356-9786

Link

https://zjps.journals.ekb.eg/article_184798.html

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https://zjps.journals.ekb.eg/service?article_code=184798

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5

Type

Original Article

Type Code

862

Publication Type

Journal

Publication Title

Zagazig Journal of Pharmaceutical Sciences

Publication Link

https://zjps.journals.ekb.eg/

MainTitle

MICROBIAL DEGRADATION OF THE MICROBIAL POLYESTER CO-POLYMER POLY HYDROXY BUTYRATE/VALERATE

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Article

Created At

22 Jan 2023