Two selective, sensitive, reproducible methods for the rapid simultaneous determination of vitamin A palmitate , E (α -tocopherol) acetate and D₃ are described. The HPLC methods depends on a simple rapid isocratic and reserved phase C-18 for identification and quantitation of vitamin A,E and D₃ in synthetic water dispersible liquid and solid mixtures, as well as a market liquid dosage form containing specific emulsifier and additives. The HPLC determination is linear over a wide range with a lower limits of quantitation of 50 ng/ml, 10 ng/ml for vitamin, A, E and D₃ , respectively.
The spectrodensitometric method provides a rapid and precise method for the separation and quantitation of the three vitamins when present in mixtures or even in a pharmaceutical dosage form. It is linear over a range of 1-25 ng/spot with a limit of quantitation of 1 ng/spot The described methods are suitable for quality control purposes of pharmaceutical preparations containing mixtures of vitamins with each other or with additives without interferences.