Sixty six catfish weighing about 200 gm each were used in the present study. Six fish were kept as a control, the others were classified into two groups in the present study each of 30 and were given enrofloxacin and ciprofloxacin (10%) daily for 5 successive days (5 mg/kg B.wt.) in aquarium respectively. Blood and tissue samples were taken from six fish from each group at 24,48,72,96 and 120 hours for studying some biochemical and hormonal parameters as well as their residues. The residues of these drugs were detected microbiologically using Bacillus subtilis organisms on nutrient agar medium. The effects on some biochemical parameters as well as reproductive hormones were also investigated. It was observed that enrofloxacin and ciprofloxacin residues could be detected in muscle samples up to 96 hours and 120 hours respectively (1.1 mm and 2.2 mm). The residues could not be detected after 120 hours for both drugs. Enrofloxacin and ciprofloxacin induced a significant decrease in serum total proteins. The drugs also showed a significant increase in serum creatinine, AST and ALT blood urea nitrogen. Testosterone and oestrogen levels decreased significantly after 4 days from exposure to enroflxacin and ciprofloxacin. The gonadotropins did not show any significant difference . It is concluded that residues of enrofloxacin and ciprofloxacin lasted for 6 days in fish muscles. Prolonged exposure to these drugs resulted in a decrease in the oestrogen and testosterone levels while gonadoprins were not altered.