An analytical method for the determination of ofloxacin or ciprofloxacin hydrochloride in pharmaceutical preparations by high performance liquid chromatography was developed. Piperazine, triethylformate, 3-chloro-4-fluoroaniline and 1,3-dichloro-4-fluorobenzene do not interfere with the analysis of ofloxacin. Also, cyclopropylamine, piperazine hexahydrate, ethylene diamine ciprofloxacin analog, 3- chloro-4- fluoroaniline, 1,3-dichloro-4-fluorobenzene and fluoroquinolonic acid do not Haterfere with the analysis of ciprofloxacin Salicylic acid was used as internal standard and the mobile phase consisted of a mixture of 35% methanol in 0.05 M sodium dihydrogen phosphate and the pH adjusted to 2.8 at flow rate 1 ml min^-1.
The chromatographic separation was achieved on Lichrospher 60-RP column with ultraviolet detection at 274 nm. The BOcratic system was operated at ambient temperature and required 8 min of chromatographic time. The method was applied to Tanvid^® tablets and Ciprinol ^® ampoules with relative standard deviation 0.75 and 1.62, respectively.