The composition of the hydrodistilled essential oils obtained from the leaves of two cultivars of Annona squamosa L. (Balady and Abdel-Razik cultivars) were analyzed by gas chromatography-mass spectroscopic technique (GC- MS). The study resulted in the identification of 18 and 26 components, comprising 89% and 92.7% of the oils of the Balady and Abdel-Razik cultivars, respectively. Results showed evident qualitative and quantitative differences between the two oils. Both oils were rich in sesquiterpene hydrocarbons (40.3% and 42.2%, respectively). Sesquiterpene alcohols were higher in the Balady (35.0%) than in Abdel-Razik (10.9 %) cultivar, while monoterpene alcohols were found in a higher percentage in Abdel-Razik (21.4 %) than in the Balady cultivar (9.5 %). The two oils exhibited remarkable wide spectrum antibacterial effects, but no antifungal activity.