Ofloxacine was labeled with ^99mTc. The labeling reaction was carried under the specified conditions. The yield of the labeled ofloxacine was determined using TLC and HPLC and it was higher than 95% .The radioactive preparation is able to localize in both bacterial and sterile inflammations induced by Escherichia coli and turpentine oil which suggest that its accumulation due to increased blood flow together with enhanced vascular permeability. Also the freeze-dried kits were designed for direct labeling technique The solution of Sn- ofloxacine was sterilized by 0.22µm millipore filtration and dispensed in a laminar flow hood (1 ml/vial), then the vials were introduced in the lyophilizer. The process of lyophilizer was continued for 24 h. At the end of the cycle, the vials were closed under nitrogen The prepared Ofloxacine freeze-dried kits have high radiochemical purity ≥95% and it was stable for at least 8h after labeling