The essential oils of Vitex agnus-castus and its variety alba Weston cultivated in Zagazig-Egypt were analysed using GC and MIS. In this investigation, comparative study of the oils of the fruits, leaves and flowers of both plants were investigated and Compared with each other as well as testing their antimicrobial activity. About 31 components were identified. The major components were 1,8-cineol, sabinene, α-pinene, isocaryophyllene, ß-caryophyllene and bicyclogermacrene.