β-sitosterol, β -sitosterol-o-glucoside, eriodictyol and luteolin were isolated from the aqueous ethanolic extract of pea nut shells after partitioning with petroleum ether, chloroform and ethyl acetate. The identity of the four compounds were done via chromatographic and spectral analysis, as well as comparison with reported data and with authentic samples. β -sitosterol-o- glucoside is reported here for the first time from pea nut shells. The amino acids and the protein contents were determined. In addition, the antimicrobial, and the anti-inflammatory studies were done.