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178989

HIGH PERFORMANCE LIQUID CHROMATOGRAPHY AND ENZYME-LINKED IMMUNOSORBENT ASSAY (ELISA) FOR THE DETERMINATION OF COLCHICINE AND RELATED COMPOUNDS

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Last updated: 04 Jan 2025

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Abstract

An HPLC method was applied for the determination of colchicine and colchicoside in the methanolic extract of the seeds of Colchicum autumnale L. during different stages of seed maturity. The method is carried out isocratically on a reversed-phase column using H2O-CH3CN (95:5) +100 µl H3PO4. The detection limits were 0.10 and 0.05 µg for colchicine and colchicoside, respectively and the recoveries (99.2 ± 1.1%) were high and quantitative. Also, a highly specific and sensitive enzyme-linked immunosorbent assay (ELISA) was developed for detection and quantitative determination of colchicine, its structurally related compounds (colchicinoids) in seeds of C. autumnale and suspension culture of Colchicum variegatum L. A combined experiment (HPLC- ELISA) was performed for detection of colchicine and some other colchicinoids in C. autumnale seeds extract.

DOI

10.21608/zjps.2002.178989

Authors

First Name

Taha

Last Name

Sarg

MiddleName

-

Affiliation

Department of Pharmacognosy, Faculty of Pharmacy, University of Zagazig, Egypt.

Email

tmsarg@pharmacy.zu.edu.eg

City

-

Orcid

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First Name

Meinhart

Last Name

Zenk

MiddleName

-

Affiliation

Institute of Pharmaceutical Biology, Munich University, Munich, Germany

Email

meinhart.zenk@yahoo.com

City

-

Orcid

-

First Name

Sameih

Last Name

El-Dahmy

MiddleName

-

Affiliation

Department of Pharmacognosy, Faculty of Pharmacy, University of Zagazig, Egypt.

Email

siel-dahmy@pharmacy.zu.edu.eg

City

-

Orcid

-

First Name

Afaf

Last Name

Abdel-Ghani

MiddleName

-

Affiliation

Dept. of Pharmacognosy, Faculty of Pharmacy, Zagazig University, Zagazig , Egypt

Email

asabdel-ghani@pharmacy.zu.edu.eg

City

-

Orcid

-

First Name

Maged

Last Name

Abou-Hashem

MiddleName

-

Affiliation

Department of Pharmacognosy, Faculty of Pharmacy, Zagazig University, Zagazig, Egypt

Email

mmabou-hashem@pharmacy.zu.edu.eg

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-

Orcid

-

Volume

11

Article Issue

2

Related Issue

24757

Issue Date

2002-12-01

Receive Date

2002-09-29

Publish Date

2002-12-01

Page Start

64

Page End

70

Print ISSN

1110-5089

Online ISSN

2356-9786

Article File

ZJPS_Volume 11_Issue 2_Pages 64-70.pdf

PDF . 3.3MB

Link

https://zjps.journals.ekb.eg/article_178989.html

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https://zjps.journals.ekb.eg/service?article_code=178989

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Type

Original Article

Type Code

862

Publication Type

Journal

Publication Title

Zagazig Journal of Pharmaceutical Sciences

Publication Link

https://zjps.journals.ekb.eg/

MainTitle

HIGH PERFORMANCE LIQUID CHROMATOGRAPHY AND ENZYME-LINKED IMMUNOSORBENT ASSAY (ELISA) FOR THE DETERMINATION OF COLCHICINE AND RELATED COMPOUNDS

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Article

Created At

22 Jan 2023