Phytochemical investigation of the chloroform extract of Verbena supina L. afforded ursolic acid, oleanolic acid, 3β, 24-dihydroxy-urs-12-en-28-oic acid and 3 β,24-dihydroxy-olean-12-en-28-oicacid.The ethyl acetate extract yielded the pheny1propanoid acteoside. The isolated materials were identified on the bases of chromatographic spectroscopic (UV, JR, ¹H and ¹³C-NMR and MS) evidences. Chloroform extract, ursolic acid and 3β,24-dihydroxy-urs-12-en-28-oic acid exhibited potent anti-inflammatory activities in comparison with hydrocortisone using carrageenan- induced hind paw edema model.