Dietary soybean has been reported to possess many of health benefits. Soybeans were found to contain potential antioxidants. The effect of soybean on paraquat-induced oxidative stress was investigated in rats. Four groups of male Albino rats were fed for two weeks the experimental diet which contained casein as control group and soybean dietary groups. Two groups of the treated rats were supplemented with 0.025% paraquat for induction of oxidative stress Soybean diet showed significant decrease in serum and hepatic Malondialdelyde (MDA) as an index of lipid peroxidation and oxidative stress. Also there were significant decrease in total serum cholesterol and triacylglycerols. Moreover, soybean diet showed an increase in blood glutathione peroxidase GSH-PX, hepatic glutathione (GSH) and catalase (CAT) levels, besides an inhibition of blood superoxide dismutase (SOD). So, the present data confirmed that, dietary soybean affected the antioxidant status of the body and reduced paraquat induced oxidative stress.