Phytochemical investigation of both the chloroform and ethyl acetate extracts of Lonicera sempervirens led to the isolation of seven compounds identified as betulin 1, a new flavonoid compound named (lonisemperin) scutellarein-(6-O-3')-acacetin 2, luteolin 3, β-sitosterol 3-O-β -D-glucoside 4, from the chloroform extract and acacetin-7-O-β -D-glucoside 5, luteolin-7-O-β -D-glucoside 6 and apigenin-7-O- β-D-neohesperidoside 7, from the ethyl acetate extract. The isolated compounds were identified according to their chemical and spectral data. The antifungal activity of the extracts and some of the isolated compounds were performed using Aspergillus parasiticum which secrets aflatoxines.