Pharmacognostical studies on zizyphus lotus (L.) Lam; was carried out to identify and differentiate the plant organs. Phytochemical screening revealed that the plant rich with carbohydrates and/or glycosides, volatile substances, saponins, flavonoids, phenolics, steroids and/or triterpenes, and alkaloids and/or nitrogenous bases. The biological studies showed that 70% alcohol extract had promising antimicrobial activity. By examination of the LD₅₀ and analgesic effect after oral administration of various doses of extract 100, 200 and 300 mg/kg body weight, the LD₅₀ of 70% alcohol extract of Zizyphus lotus L. (Lam ) is 12.88 g /kg (mice) with confidence limit of 10.75 g /kg (mice) and 15.44 g /kg (mice). All tested extracts showed significant analgesic activity in all times of experiments except at 20 min. In conclusion.the tested extract can be used safely for analgesic purposes.