The study aimed to update the resistance profiles of some uro-pathogenic E. coli isolates towards some antibiotics and to test the resistance of the multiple resistant isolates towards some commonly used biocides. One hundred E.coli isolates were recovered from inpatients and outpatients clinical cases of uncomplicated urinary tract infections. Antibiotic sensitivity against cefotaxime, ciprofloxacin, tetracycline, rifampicin, chloramphenicol, gentamicin, imipenim , cefuroxime , ampicillin and erythromycin was determined by agar diffusion. About 20% of these isolates were found to be multiple antibiotic resistant.On the other hand, about 7% of isolates were completely susceptible to all tested antibiotics. From all isolates,58% were found to be resistant to at least one antibiotic , 15% were resistant to two antibiotics, where only 1% was found to be resistant to all used antibiotics. Calculated MIC₅₀ and MIC₉₀ demonstrated that the most effective antibiotic was cefotaxime with MIC₅₀ & MIC₉₀ of 8 and 32 µg/ml, respectively. MIC values of some biocides (cetrimide, chlorhexidin, chlorocresol and phenylmercuric nitrates) for the multiple resistant isolates were determined and a correlation was found between MIC values of some antibiotics and biocides, namely between chlorocresol and each of chloramphenicol and tetracycline, and between cetrimide and each of gentamicin and tetracycline.