The aim of the present study was to determine susceptibility pattern among Staphylococcus aureus clinical isolates from ICUs of Mansoura University Hospitals and to investigate the prevalence of MRSA in order to make continuous monitoring to the action of anti-staphylococcal drugs in Egypt and to initiate the treatment with the appropriate antibiotic to avoid failure of treatment due to resistance. A total of 100 clinical isolates were collected and identified as Staphylococcus aureus from blood samples of patients in ICUs of Mansoura University Hospitals. The isolates were tested for antimicrobial susceptibility against 18 different antibiotics by using disk diffusion method. Resistance rate for linezolide and vancomycin were 0%. The most effective antibiotic were clindamycin and sulphamethoxazole-trimethoprim their resistant rate were 25% and 19% respectively. Forty-five (45%) of isolates were methicillin resistant (MRSA). It is recommended by the health authority to limit the further increase of antimicrobial resistance among Staphylococcus aureus by declining the rational treatment regimen.