This study aimed for the molecular detection of TEM and SHV genes in Extended-Spectrum beta-lactamases (ESBLs) clinical isolates of Enterobacteriace (Eshericichia coli, Klebsiella pneumoniae and Proteus spp.). ESBLs are one of the main cause of resistance of Enterobacteriace family to beta-lactam antibiotics. The susceptibility of isolates to different beta-lactam and non-beta lactam antimicrobial agents was performed to identify the proper antimicrobial chemotherapeutics for treatment of infections in case of development of resistance to Extended spectrum beta-lactams. Ninety clinical isolates belong to Enterobacteriacae family (54 E. coli, 28 Kl. Pneumonia and 8 Proteus spp) recovered from 220 different clinical specimens (urine, blood, pus and sputum). Thirty (33.3%) out of 90 isolates were ESBLs producers. The ESBLs producers were more frequent among E. coli isolates (40.7%), followed by Proteus (25%) and klebsiella pneumoniae (21.4%). Susceptibility testing to Beta-lactams and non beta-lactams was performed by disk diffusion method to all isolates. The bla TEM gene was identified in 14 of E. coli isolates at 504 bp. The blaSHV-625 gene was identified in two isolates of Klebsiella pneumoniae at 625bp. Meanwhile blaSHV-1,107 was not detected in any clinical isolates.