Accurate, sensitive, robust and direct isocratic Reversed Phase-HPLC methods, using Evaporative Light Scattering Detector, (ELSD), had been developed and fully validated for the detection and quantification of two non-chromophoric nutraceuticals; Alpha Lipoic Acid (ALA) and Sucralose (SUC). Chromatographic separation was achieved using C18 Zoprax, 5 μm, 15 cm column for both compounds. For ALA, mobile phase was composed of acetonitrile: 0.1 M acetic acid (60:40, v/v), adjusted at pH 2.5, with 0.6 mL/min flow rate. For SUC, acetonitrile: deionized water (70:30, v/v) with 1 mL/min flow rate was used. Temperatures of drift tube, column, and spray chamber have been set to be 40°C and 30°C respectively during both assays. All varying chromatographic parameters were studied carefully. ALA and SUC had eluted at 4.81±0.02 min and 1.70±0.01 min, respectively. The obtained exponential ELSD responses, for the two developed methods, were linearly modeled using logarithmic transformation. Good Linearities had been achieved over the concentration ranges of 100-750 ppm and 16-500 ppm for ALA and SUC estimation respectively. The suggested methods disclosed excellent precision and accuracy levels. All validation parameters were fulfilled according to the USP elements and ICH guidelines. Satisfactory percentages of recovery (>97%) resulted upon methods application for the assay of each dietary supplement in its pharmaceutical formulation. Comparative statistical study was conducted between the proposed HPLC/ELSD methods and the reported HPLC/UV method for ALA and reported HPTLC method for SUC. Student's t-tests and F-variance ratios for both methods had resulted in satisfactory values.