This study evaluated the effect of three different anti-bacterial cleansing agents on microshear bond strength to dentin with self etching bonding agent. Materials&Methods; Flat dentin surface of 120 molars was prepared, samples was divided into four main equal groups (30 teeth each) according to the type of disinfectant pretreatment; group(D1) Chitosan nanoparticles (CNPs), group (D2) Cetylpyridinum chloride (CPC), group (D3) Pomegranate peel extracts (PPE) and group (D4) Control (no pretreatment). Self-etch adhesive system G-aenial bond and Kalore resin composite was applied. Microshear bond strength testing was done using universal testing machine. Result; the highest mean microshear bond strength (27.139 ± 2.72 MPa) was recorded for those dentin specimens treated with (CNPs)after 1day storage time while the lowest mean shear bond strength (15.33 ± 2.44 MPa) was recorded for those dentin specimens treated with (PPE) after 3 months storage time Conclusions; (CNPs) application before bonding with self-etch adhesive preserve bond strength over time.