This study evaluated the effect of three different anti-bacterial cleansing agents on tensile bond strength of composite to dentin. Materials Methods; Flat dentin surface of 12 molars was prepared, the teeth were divided into four main equal groups according to surface pretreatment (three types of disinfectant pretreatment and one control); group chlorhexidine digluconate (CHX), group Cetyl pyridinium chloride (CPC), group Pomegranate peel extracts (PPE) and group Control (no pretreatment). Then, nanocomposite was built up bonded to the treated surfaces with an adhesive system. Then, the teeth were sectioned perpendicular to composite–teeth interface to obtain 120 rectangular sticks, and the tensile bond strength testing after storage in water for one day, one month, and three months. Finally, (random specimens) one specimen from each group of storage time (one day, and three months) were used for scanning electron microscope examination to evaluate the failure mode and the effect of various treatments on the dentin surface topography. Result; The highest tensile bond strength (27.139±2.72 MPa) was recorded for specimens treated with (CHX) after 1-day storage time while the lowest tensile bond strength (15.33±2.44 MPa) was recorded for specimens treated with (PPE) after 3 months storage time. Conclusions; CHX application before bonding with adhesive preserve bond strength over time. Using CHX, CPC and PPE have no adverse effect on the bond strength. Storage in water has a highly significant adverse effect on the bond strength to all groups especially with treated with PPE and with no surface treatment.