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Evaluation of the effects of Botulinum Neuro Toxin A (BoNTA) on Submandibular Salivary Gland in Rats by histological examination and semi-quantitative scoring method

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Last updated: 04 Jan 2025

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Abstract

Introduction: Botulinum toxin A (BoNTA) has been used for treating hyperfunction of various glands such as sweat, lacrimal, and salivary glands. However, the long-term histological sequences are largely unknown. Objectives: The present study is to evaluate the histological and ultrastructural effects of BoNTA on submandibular salivary gland (SSG). Methods: Sixty male albino rats received 0.1 ml of either saline (control group) or BoNTA (BoNTA group) injection in the right SSGs. The rats were terminated at 2, 4 and 12 weeks after the injection. The harvested SSGs were embedded and sectioned at 4-5µm and stained with H&E for histological study. Results: All control SSGs showed normal acinar cells with rounded nuclei and regular striated ducts (SD) with characteristic basal striations. Compared with these features, 2-week BoNTA-injected SSGs showed loss of spherical fashion and basal striations in serous acini and SD respectively, and the cell boundaries were not clear. In 4-week SSGs, some acini and ducts lost their spherical fashion and, in some areas, these structures disappeared. However, all 12-week BoNTA-injected SSGs seemed to have similar structures to those of control SSGs. By using scoring system for semi-quantifying the histological structural changes of BoNTA-injected SSGs, 2- and 4-week BoNTA-injected SSGs showed significantly higher scores as compared with their control counterparts. However, no significant score differences were found between 12-week BoNTA-injected and control SSGs. Conclusions: Although application of BoNTA results in significant changes in histological structures of SSGs, these detrimental effects seems to be transient, and the major recovery occurs after 3 months. Thus, BoNTA can be used for treating SSG hyperfunction.

DOI

10.21608/ajdsm.2018.71695

Keywords

Botulinum toxin A, Submandibular salivary glands, histology, semi-quantitative scoring, Rats

Authors

First Name

Hany

Last Name

Sherif

MiddleName

Abdel-Hamied

Affiliation

Professor of Oral Biology, Al-Azhar University, Cairo, Egypt.

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Orcid

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First Name

Abdelnaser

Last Name

Esmail

MiddleName

Abdelmawla

Affiliation

Assist. Professor of Oral Biology, Assiut University, Egypt.

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City

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Orcid

-

First Name

Mohamed

Last Name

Abdelfattah

MiddleName

Yehia

Affiliation

Dept. Oral Biology, Assiut University, Egypt.

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Orcid

-

First Name

Zi-Jun.

Last Name

Liu

MiddleName

-

Affiliation

Professor of Orthodontics, University Washington, Seattle, USA

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-

City

-

Orcid

-

First Name

Wael

Last Name

Abouzid

MiddleName

-

Affiliation

Professor of Basic Dental Science, National Research Centre, Cairo, Egypt.

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Orcid

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Volume

21

Article Issue

5

Related Issue

10905

Issue Date

2018-12-01

Receive Date

2020-02-17

Publish Date

2018-12-01

Page Start

511

Page End

518

Print ISSN

1110-6751

Online ISSN

2682-3314

Article File

AJDSM_Volume 21_Issue 5_Pages 511-518.pdf

PDF . 1.2MB

Link

https://ajdsm.journals.ekb.eg/article_71695.html

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https://ajdsm.journals.ekb.eg/service?article_code=71695

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Original Article

Type Code

848

Publication Type

Journal

Publication Title

Al-Azhar Journal of Dental Science

Publication Link

https://ajdsm.journals.ekb.eg/

MainTitle

Evaluation of the effects of Botulinum Neuro Toxin A (BoNTA) on Submandibular Salivary Gland in Rats by histological examination and semi-quantitative scoring method

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Article

Created At

22 Jan 2023