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Evaluation of Antibiofilm Efficacy of Nanoparticle Agents Using Photodynamic Therapy; An In Vitro Study

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Last updated: 04 Jan 2025

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Abstract

Objective: This study evaluated the antibiofilm efficacy of nanoparticle agents using photodynamic therapy. Methods: A total of Ninety dentin section blocks were used in this study. Dentin section blocks were prepared to be (4×4×1 mm) (Length× Width × thickness) respectively. Each dentin section was placed in 1.5 mL polypropylene tube filled with brain heart infusion (BHI) broth then underwent sterilization using autoclave at 121°C for 20 minutes and the sterilization was confirmed using bacterial count test. Cultivation of Standard strains of E. Faecalis ATCC 29212 and Staph. Epidermidis ATCC 12228 were prepared then inoculated on dentin section blocks and incubated for 3 weeks at 37°C in incubator (The medium was changed every 3 days). The specimens were grouped into 4 groups according to Final irrigant rinse were used: G1: final rinse using chitosan nanoparticles (CSnps)., G2: final rinse using Rose Bengal dye (RB), G3: final rinse using chitosan nanoparticles conjugated with Rose Bengal dye (CSRBnps) and G4: control group. Each group was activated using a diode laser 635 nm for 5 minutes at energy fluence 30 J/cm2. Then, the effect of tested final rinsing on multispecies biofilm was assessed with Confocal Laser Scanning Electron Microscope (CLSM) and data were statistically analyzed. Results: With regard to the antimicrobial effectiveness of tested irrigants, the highest mean percentage of dead bacteria was found in {CSRBnp (Group 3)} (88.38 ± 5.45) followed by {Csnp (Group 1)} (75.73 ± 5.15) followed by Rose Bengal dye (Group 2) (59.43 ± 8.14). The least mean percentage of dead bacteria was found in Control group (Group 4)} (7.25 ± 1.12). With a statistically significant difference between groups where (p<0.001). Conclusion: Functionalization of chitosan nanoparticles with Rose Bengal dye is a potent antibiofilm strategy for disinfection of the root canal system.

DOI

10.21608/ajdsm.2018.71516

Authors

First Name

Abdelrahman

Last Name

A. Hamouda

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Affiliation

Department of Endodontics, Faculty of Dental Medicine, Boys, Cairo, Al-Azhar University

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First Name

Mohamed

Last Name

H. Morad

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Affiliation

Professor of Microbilogy, The Regional Centre for Mycology and Biotechniology, Al-Azhar University

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First Name

Ahmed

Last Name

M. Farghaly

MiddleName

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Affiliation

Associate Professor of Endodontics, Faculty of Dental Medicine, Boys, Cairo, Al-Azhar University

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First Name

Taher

Last Name

M. Islam

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-

Affiliation

Professor of Endodontics, Faculty of Dental Medicine, Boys, Cairo, Al-Azhar University

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Volume

21

Article Issue

3

Related Issue

10890

Issue Date

2018-07-01

Receive Date

2020-02-17

Publish Date

2018-07-01

Page Start

197

Page End

203

Print ISSN

1110-6751

Online ISSN

2682-3314

Article File

AJDSM_Volume 21_Issue 3_Pages 197-203.pdf

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https://ajdsm.journals.ekb.eg/article_71516.html

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https://ajdsm.journals.ekb.eg/service?article_code=71516

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Type Code

848

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Journal

Publication Title

Al-Azhar Journal of Dental Science

Publication Link

https://ajdsm.journals.ekb.eg/

MainTitle

Evaluation of Antibiofilm Efficacy of Nanoparticle Agents Using Photodynamic Therapy; An In Vitro Study

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Article

Created At

22 Jan 2023