This research was designed to evaluate the effect of resin infiltration material (Icon) and remineralizing agents casein phosphopeptide- amorphous calcium phosphate (CPP-ACP) and Nano hydroxyl apatite (n-HA) on surface roughness and microhardness of enamel white spot lesion. A total number of 120 sound non-carious human anterior teeth were collected. The labial surfaces of all specimens are treated by 37% phosphoric acid for one minute to create artificial enamel white spot lesion. The teeth were divided into two main groups (60 teeth each); Group H: for assessment of enamel microhardness and Group R: for assessment of surface roughness. Each main group was further divided into four equal subgroups (15 teeth each) according to the treatment applied as follows: Subgroup (C): control, Subgroup (P): CPP-ACP, Subgroup (I): Icon, Subgroup (N): Naohydroxyapatite. Each subgroup was further subdivided into three equal divisions (5 teeth each) according to the storage period in artificial saliva; one week (1W), four weeks (4W) and eight weeks (8W). For assessment of microhardness; the enamel white spot lesion specimens in the four different subgroups were examined three times (after one week, four weeks and eight weeks from the first time of treatment application) by Digital Display Vickers Micro-hardness Tester. The results of microhardness revealed that the subgroup (N) group was provided the highest hardness mean value, followed by subgroup (I), then subgroup (P) while subgroup (C) showed the lowest microhardness mean value. For assessment of surface roughness; the enamel white spot lesion specimens in the three different subgroups were examined three times (after one week, four weeks and eight weeks from the first time of treatment application) by non-contact profilometer. The results of surface roughness revealed that the subgroup (P) was provided the highest roughness mean value, followed by subgroup (C) group while subgroup (I) showed the lowest surface roughness mean value.