A pot experiment was carried out on lettuce(Lactuca sativa L.) for growing 2021 season to study the effect ofvarious water sources i.e.,fresh water (FW),agricultural drainage (ADW), sewage (SW) andindustrial drainage (IDW) after treated with moringaseed extract (MSE) on remove water and soils contamination and its effect on yield and heavy metals content of lettuce plants.The lowestvalues of SSP, SAR, SCAR, RSC, RSBC, PI, PS, KR and MAR were obtaindin fresh water followed byindustrial drainage water, sewage water and agricultural drainagewater when treated with MSE. The classification ofall water sources indicates before treated with MSE of high salinity-low sodicity(C4S1) and moderate low sodicity (C2S1) after treated with MSE. Addition of MSE to water sourecesreduced water turbidity, NO₃-N, BOD, COD, DO and heavy metals compared with untreated water. The highest values of fresh and dry weight, N, P and K- uptake of lettuce plants obtaintedindifferent water sourcestreatedwith MSE.Addition of MSE to different water sources gave the lowest accumulation ofPb, Cd, Ni, Fe, Cu, Zn and Mnin soiland lettuce plantscompared to untreated water.