The present study carried out to investigate the effect of bile salts (BS) on some blood hematology and intestinal histological changes of broiler chickens. For this purpose, 120 chicks of Ross 308 one day were randomly distributed to four treatment groups with 5 replicates, each 6 birds/replicate. The first group was fed on basal diet only and served as control. The second, third and fourth groups were fed the basal diet plus 0.5, 1 and 1.5 ml bile salts/kg diet, respectively. The results cleared that the addition of 1.0 ml BS/kg in broiler chicks' diets increased significantly (P<0.05 or P<0.01) blood hemoglobin concentration. Histological status of small intestine significantly (P<0.01) affected by addition (BS) to broiler diet. Whereas, villas length and width significantly (P<0.01) increased in groups treated with different levels of BS than control group. From these results, it can be concluded that bile salts had a positive impacts on hemoglobin concentration, morphology and histological changes of small intestine in broiler chicks.