The current work aimed to study the effects of dietary grape seed extract (GSE) supplementations at different levels on the productive performance of aged two local strains (Matrouh and Inshas) laying hens. A total number of 120 Inshas and 120 Matrouh strain birds at 48-wks-old were used in this experiment up to 64weeks.of age in a 2 × 4 factorial arrangement. All birds in each strain were individually weighed and randomly divided into four equal experimental groups (thirty laying hens of each) with three replicates (ten laying hens each) with almost similar initial average body weight. Within each strain, the 1st group was fed the basal diet without supplementation while, the 2nd, 3rd, and 4th groups were fed the basal diet supplemented with 600, 1200 and 1800 mg GSE/kg diet, respectively. The obtained results of the present work could be summarized as follows: Hens of Inshas strain had significantly (P≤0.05) heavier egg weight greatest egg production and egg mass when compared with those of Matrouh at all intervals studied. Hens fed diet supplementation with 1200mg GSE/Kg diet record highest egg production and egg mass. The interaction between strain and dietary GSE supplementation levels was significant (P≤0.01) on egg weight at all ages studied except during the interval of 56-60 weeks of age. Hens of Inshas had significantly lower values of FCR than those of Matrouh during 48 – 52 weeks of age. Hens received diet contained 1200 mg GSE/ kg diet showed significantly the best FCR at intervals of 52-56, 56-60, 60-64 and 48-64 weeks of age when compared with the control and other treatment groups. The interaction between strain and dietary GSE supplementation levels was significant on FCR at all ages studied. During the whole period (48-64 weeks of age), hens of Inshas fed diet supplemented with 1200 mg GSE/ kg diet showed the best feed conversion ratios in comparison with the control and other treatment groups. Conclusively, it is could be concluded that supplementation of GSE into layer diet caused beneficial effects on their productive performance.