This study aimed to determine the effect of cefepime on different liver and kidney functions of healthy rabbits (35days/1kg) were injected with singular intramuscular dose once daily for 5 successive days with 75 mg/kg body weight. Twenty-four healthy rabbits of the same sex were divided into two groups (n=12). First group was injected with distilled water (control) and second group was injected with 75 mg/kg once daily for 5 successive days. Blood samples were taken at (1^st, 3^rd, 7^th and 14^th day) without anticoagulant, centrifuged and frozen. Samples were analyzed and the results indicated that there was non-significant effect on liver functions as there was no change in each of the serum AST, ALT, ALP, total protein, and total albumin. The results indicated also that there was insignificant effect of cefepime on kidney functions, in terms of insignificant difference of serum urea and concentration and creatinine levels, meaning the safety of the drug on public health.