The present study was carried out to evaluate the relative biological activity of six treatments. Four nanoparticles (chitosan, Hydroxyapatite, chitosan hydroxyapatite and silver hydroxyapatite), one bio-agent effective microorganisms (EMs) compared to one synthetic acricide (bifenzata) were tested against the female adults and eggs of Tetranychus urticae and its predatory mite Amblyseius gossipi using standardized method of bioassay under laboratory conditions. Effect of the tested agents on the activity of carbohydrates enzymes, total lipid and total protein content were determined. The results revealed that chitosan (NPs) showed significantly adulticidal activity against T. urticae. Furthermore, EMs was the most effective treatment on the eggs of T. urticae followed by bifenzata under laboratory conditions. Therefore, nanoparticles and EMs appeared to be highly safe and selective against Amblyseius gossipi. This study suggests the possible use of nanoparticles as safe alternatives to conventional acaricides and compatible with integrated pest management practices. They might be contribute to future applications in pest control for sustainable agriculture.