This experiment was carried out during 2013 and 2014 seasons on guava seedless fruits to study the effect of spray of three bioinsecticides; Biozeid 2.5%, Pritecto 9.4% and Bioarc 6% three levels each (300, 250 and 200g/100 l water) at preharvest to control peach fruit fly during postharvest of guava fruits. Stored fruits at 8°C and RH.85-90%. Weight loss,firmness,total soluble solides, carotenoids, acidity and vitamin C, were determind. The highest effect for controlling peach fruit fly observed with Bioarc 6% treatments of guava fruits in both seasons. The results indicated that, there was lower weight loss with fruits which were treated by Biozeid 2.5% and by Pritecto 9.4%. Firmness, carotenoids and vitamin C. were significantly higher with fruits treated by Bioarc 6%, but total soluble solid was significantly higher in fruits treated by Biozeid 2.5% in both seasons. In both seasons, weight loss percentage, total soluble solid contents (TSS) and carotenoids were increased significantly by the advancing of storage periods but firmness, total acidity and vitamin C. decreased during storage.