Different concentrations (0.0, 1.0, 2.0, 4.0, 8.0 or 16.0 mg/l) of cytokinins (benzyladenine, kinetin or isopentenyladenine) were investigated in order to enhance shoot multiplication of Aglaonema commutatum. Results indicated that benzyladenine (BA) at 8.0 mg/l gave the highest number of shoots/ explant (4.08). Different concentrations (0.0, 0.5, 1.0 or 2.0 mg/l) of auxins (indoleacetic acid or naphthaleneacetic acid) combined with 8.0 mg/l BA were tested to improve shoot multiplication. NAA at 0.5 mg/l combined with 8.0 mg/l BA proved to be the best treatment for obtaining the maximum number of shoots/explant (6.70). For root formation induction, shoots obtained from multiplication stage were transferred to MS medium contained different concentrations (0.00, 0.25, 0.50, 1.00 or 2.00 mg/l) of auxins (indoleacetic acid, naphthaleneacetic acid or indole-3-butyricacid). Results showed that 1.0 mg/l of IAA or NAA were the best treatments for root induction and quality during rooting stage. Rooted shoots were acclimatized by transferring them to plastic pots containing peat moss, vermiculite, perlite or sand each alone or in different combinations among them at equal volume proportions. Perlite and peat moss at equal volume proportions seemed to be the best medium for plantlets acclimatization.