The present investigation was carried out to identity eight barley varieties using DNA fingerprinting detected by (SSR)-PCR molecular markers and SDS- PAGA protein electrophoresis. Twelve anchored SSR primers were used for fingerprinting of the eight barley varieties and 76 bands were detected, from which 56 bands (74%) were polymorphic. Giza 125 variety produced the largest number of markers. It produced 14 band markers in seven primers (SSR 1, SSR 4, SSR 5, SSR 6, SSR 7, SSR 11 and SSR 12). Giza 123 variety produced five bands in three primers (SSR 5, SSR 8 and SSR 12). Giza 127 produced four bands in three primers (SSR 5, SSR 8 and SSR 9). Giza 124 produced two bands in two primers (SSR 2 and SSR 8). Also Giza 132 variety produced two bands in two primers (SSR 9 and SSR 11). Giza 129 variety has one band marker in primer SSR 8. On the other hand, the results of protein electrophoresis revealed that, total number of bands was 13, out of which 7 bands (54%) were polymorphic. Protein electrophoresis produced four marker bands, three of them were found in variety Giza 124 and one in variety Giza 129. Existing genetic differences between varieties are of great importance from the point of view of the breeder to take advantage in barley improvement programs.