The present work was carried out at the laboratories of Hort. Dept., Agric. Fac., Zagazig University, Egypt during 2014 and 2015 seasons to improve keeping quality of gerbera cv. Rosamine cut flowers by using of pulsing, holding solutions and cold storage for several durations as well as their combination treatments. Besides, studying these treatments affects on post harvest characters and water relation characters as well as some chemical constituents in gerbera cut flowers. Pulsing solution treatments used were control distilled water (D.W.), sucrose (S) at 5 % plus 8-hydroxy quinolene sulphate (8-HQS) at 200 ppm for 18 hours, benzyladenine (BA) at 10 ppm + 5% S + 200 ppm 8-HQS for 18 hours, silver thiosulphate (STS) 1:4 mM for 20 minutes then placed in 5% S + 200 ppm 8-HQS for 18 hours, STS 1:4 mM for 20 minutes then placed in BA at 25 ppm + 5% S + 200 ppm 8-HQS for 18 hours and sodium nitroprusside (SNP) 250 ppm for 1 hour then placed in BA at 25 ppm + 5% S + 200 ppm 8-HQS for 18 hours. Furthermore, gerbera cut flowers were stored at 4±1°C for 0, 5 or 10 days. Whereas, holding solutions treatments used after storage period treatment were control distilled water (D.W.) and sucrose (S) 2.5% + 8-hydroxyquroline sulphate (8-HQS) at 200 ppm + citric acid (CA) at 150 ppm, besides to the combination treatments between them. The following data were recorded, post harvest characters (vase life(, water relations (water balance) and chemical constituents (anthocyanin content, total sugars, total nitrogen and protein percentages) as well as correlation coefficient among these characters as affected by the combination between pulsing solution,  storage periods and holding solution treatments used. The obtained results came to the conclusion that, the longest vase life of gerbera and the highest quality cv. Rosamine cut flowers were obtained by using the combination treatments between STS + S + 8-HQS for 18 hours as pulsing solution and storage at 4±1°C for 0-time and 5 days and holding solution tretments contained sucrose (2.5%) + 8-hydrox quinolene sulphat (200 ppm) + citric acid (150 ppm) compared to the other ones under study.