This experiment was conducted to study the effect of combinations between 350 mg vitamin E with 120 mg zinc/kg diet, or 0.3mg selenium/kg diet as feed additives on the reproductive performance of female Nile tilapia (Oreochromis niloticus) diet for 144 days. Growth rate, average weight and number of eggs, egg weight, Relative fecundity, hatching percentage, survival rate of the fry and the concentration of follicle stimulating hormone (FSH) and luteinzing hormone (LH) in the blood serum at the different treatments compared to the control group. The results showed that the increase in the final body weight with vitam. E+Zn and vitamE +Se treatments was 141.80±0.92 and 142.00±1.15 g and body length was 20.60±0.06 and 20.60±0.06 cm compared to the control groups (136.00±0.92g, 20.30±0.05cm, respectively. The results of spawning efficiency showed an increase in average weight of eggs with vitam. E +Zn and vitam. E +Se treatments valued as much as 2.613±0.085 and 2.643±0.018 g and the number of eggs produced 453.33±18.05and 475.67±4.05 egg/female/ spawning compared to the control group (2.043±0.074 g and 365.67±15.81 egg/female/spawning, respectively). The relative fecundity of female's recorded the highest value (3.20±0.12 and 3.35±0.03 egg/g of body weight) with vitam. E +Zn and vitam. E +Se treatments compared to the control group (2.70±0.12 egg/ g of body weight). On the other hand, the hatching percentage of eggs in treatment fed on supplementation diet with combination (vitam. E+ Zn) was higher (87.13±0.08%) than the other treatments. Fish fed diets supplemented with vitem. E+ Zn recorded the highest value of FSH, unlike the released hormone, which increased its concentration in blood fish in all treatments. The highest rate of increase in the body weight of fish larvae fed with (vitamin E +Zinc), the highest survival rate was with the fish group fed with a dietary supplement of vitam. E+ selenium).That is to say, female Nile tilapia (O. niloticus) feeding on diet combined with vitamin E, selenium and zinc had the positive effect on growth and reproductive performances in spawning season.