This study was conducted in Zagazig University, Egypt to evaluate tolerate biomarkers in faba bean against Orobanche crenata. Two faba bean genotypes were choicen, known as Msr-3 (tolerate) and Giza-716 (susceptible). Obtained results showed that tolerate genotype has high content of chlorophyll a (1.20 mg/ g fresh weight), chlorophyll b (0.69 mg/g fresh weight), total chlorophyll (1.89 mg/ g fresh weight) and carotenoid (0.97 mg/ g fresh weight). Also tolerate faba bean contained high content of componants known as major osmotically-active componants, namely total soluble sugar (0.045 mg/ml), total protein (7.85%), Na⁺ (39.16ppm), K⁺ (59.24ppm) and polar amino acids (78.54%). Tolerate faba bean also showed a high content of total phenolic compounds (348.84 ppm calculated as a Gallic acid), conjugated phenolic compounds (227.69 ppm calculated as a Gallic acid). As well as tolerate faba bean (Msr-3) has high catalase activity (0.39 mg. protein^-1).