A pot experiment was conducted in a green house in the farm of the Faculty of Technology and Development, Zagazig University, Zagazig, Sharkia Governorate, Egypt to study the response of wheat plant to application of N-fertilization from different N- sources i.e., ammonium sulphate (AS) and compost (CO), different nitrogen rates and their combinations as well as bio fertilization from different sources i.e, microbien (Mic.) and compost tea (CT) on the production of wheat grown on a sandy and calcareous soilsduring winter season 2014/ 2015 on dry matter yield(DM), yield components, N content and uptake, grain quality and nitrogen use efficiency, (NUE). The obtained results could be summarized as follows: application of AS solely or in a combination with biofertilizers showed the greatest values of DM yield compared to CO addition or control treatment. Grain protein content significantly increased as affected by N-rate and bio fertilization. The value of NUE was the greatest due to the addition of 50 mg N as AS kg^-1 soil solely and in a combination with compost tea and microbien. In the calcareous soil, application of 100 mg N as AS kg^-1 soil combined with (CT+ Mic.) was the most effective on wheat N-uptake at booting and straw at maturity stage compared to other treatments. Similar trend for N-uptake was observed in sandy soil.