This study was carried out during 2014 and 2015 seasons in a private plastic green house at Belbies District, Sharkia Governorate, Egypt in an attempt to replace the traditional olive cutting rooting synthetic growth regulators by some auxin releasing natural extracts. Cuttings of Picual olive cv. were prepared on November of each season (about 15 cm long and7-9 mm in diameter), cutting were dipped in concentrations of indole-3-Butyric acid (IBA) at 4000 ppm and naphthalene acetic acid (NAA) at 500 ppm solution for 5 seconds or soaked in the natural extracts for half hour. The results revealed that the maximum rooting percentage (81.44 and 85.60%), number of roots/cutting (14.84 and 14.90 root/cutting), shoot number/ transplant (2.74 and 2.17 shoot/plant), leaf number/plant (6.49 and 6.61 leaf/plant) and bud sprouting percentage (48.46 and 48.14 %) respectively were recorded for cuttings dipped in IBA at 4000 ppm in comparison with those dipped in moringa extracts, which gained the lowest values of the considered parameters during the two seasons. The highest cutting survival percentages were recorded for IBA and NAA treatments, garlic at 10 or 20%, liquorice at10 g and algae at 2.5 or 5 cm in both seasons without significant differences between them. Total carbohydrates percentages in the roots and shoots were gradually increased with the advance in planting months, and reached the highest values after seven months, but total soluble phenols in the roots were decreased after six months then increased in the seventh month in both seasons. The algae, garlic and liquorice treatments were considered the best natural extracts compared to yeast and in turn can be used as an alternative of growth regulators for rooting Picual olive cuttings.