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48574

STUDIES ON FUNGI ASSOCIATED WITH PEA SEEDS AND THEIR EFFECT ON GERMINATION AND SOME SEED CHARACTERS

Article

Last updated: 04 Jan 2025

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Abstract

Seed borne fungi of 45 pea seed samples of Master-B cultivar were examined. These samples were collected from main production area of Egypt. A total number of 28 species, representing 19 genera of fungi was isolated and identified from pea seeds. The agar plate method was more accurate for detection of the most associated seed borne fungi compared with blotter method. Test tube agar method of 50 seeds from each of Master-B, Entsar 1, Entsar 2 and Sugar gum cultivars revealed incidence of 10 species, representing 8 fungi genera. These fungi were isolated from different seedling parts. Scanning electron microscopy (SEM) was used to examine seed borne fungi in dry seed surface of two pea cultivars Cambados (curly) and Oregon sugar (smooth).The curly cultivar hosted more fungi than the smooth one. Six categories of discoloration pea seeds were investigated using agar plate method. A total of 27 species, representing 19 genera were isolated and identified from pea seeds with different color categories. The effect of discoloration on seed characters and germination were examined. Discoloration of deteriorated seeds was associated with decreased total protein, total phenols, weight of 1000 dry seeds and seed germination percentages comparing with healthy ones. On the contrary, moisture contents in healthy seeds recorded lower percent compared with all discoloration seed categories and insect infection.

DOI

10.21608/zjar.2018.48574

Keywords

Pea-seed borne fungi, blotter method, test tube agar method, pea seed discoloration, scanning electron microscopy (SEM)

Authors

First Name

Mahmoud

Last Name

Youssef

MiddleName

A.A.

Affiliation

Seed Pathol. Res. Dept., Plant Pathol. Res. Inst., ARC, Giza, Egypt

Email

mmelhakeem@yahoo.com

City

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Orcid

-

First Name

A.

Last Name

Aly

MiddleName

Z.

Affiliation

Plant Pathol. Dept., Fac. Agric., Zagazig Univ., Egypt

Email

-

City

-

Orcid

-

First Name

M.

Last Name

Tohamy

MiddleName

R.A.

Affiliation

Plant Pathol. Dept., Fac. Agric., Zagazig Univ., Egypt

Email

-

City

-

Orcid

-

First Name

M.

Last Name

Ghonim

MiddleName

I.

Affiliation

Seed Pathol. Res. Dept., Plant Pathol. Res. Inst., ARC, Giza, Egypt

Email

-

City

-

Orcid

-

Volume

45

Article Issue

4

Related Issue

7553

Issue Date

2018-07-01

Receive Date

2018-03-07

Publish Date

2018-07-30

Page Start

1,291

Page End

1,308

Print ISSN

1110-0338

Link

https://zjar.journals.ekb.eg/article_48574.html

Detail API

https://zjar.journals.ekb.eg/service?article_code=48574

Order

8

Type

Original Article

Type Code

842

Publication Type

Journal

Publication Title

Zagazig Journal of Agricultural Research

Publication Link

https://zjar.journals.ekb.eg/

MainTitle

STUDIES ON FUNGI ASSOCIATED WITH PEA SEEDS AND THEIR EFFECT ON GERMINATION AND SOME SEED CHARACTERS

Details

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Article

Created At

22 Jan 2023