This study was conducted to investigate the effect of Somali red sesame oil derived from red sesame seeds on liver function enzymes including, Alanine aminotransferase (ALT) and Aspartate aminotransferase (AST) and lipid profile including total cholesterol (TC), low density lipoprotein (LDL), high density lipoprotein (HDL) and triglycerides (TG) in male albino rats fed on fatty diet. Twenty one rats were randomly divided into equal three groups of seven animals each for 30 days as follows: The first group (control) was fed on basal diet and water, the second group was fed on fatty diet and the third group was given orally red sesame seed oil daily dose (1ml/100-120 g of body weight). Blood samples was collected in initial time and after 30 days of treatment, serum was separated for determination of liver functions and lipid profile. Results indicated that oral intake of sesame oil at dose 1 ml for 30 days (group III) decreased the serum levels of liver enzymes (AST) and (ALT) and lowering (TC), LDL and (TG) while, caused markedly increases in HDL values when compared to the control (positive group). Finally, it could be seen from the preceding results that, the utilization of the red sesame seed oil is safe for human health. Also, it can be assumed that the oil under study proved to be a prophylactic agent against hyperlipidemia.