The susceptibility of three different field strains collected from Beni Suef, Monufia and Beheira Governorates of the pink bollworm, Pectinophora gossypiella (Saunders) (Lepidoptera: Gelechiidae) to chlorpyrifos, profenofos, alpha-cypermethrin, esfenvalerate, lambda-cyhalothrin, emamectin benzoate, spinetoram and spinosad was evaluated by bioassay tests  in Central Agricultural Pesticides Laboratory. The results indicated that spinosad was the most toxic to all strains studied and in all tested seasons, 2017, 2018 and 2019. Its LC₅₀ values were 31.6, 40.0 and 67.2 mg/L in 2017, 60.0, 69.4 and 47.7 mg/L in 2018 and 42.0, 27.8 and 20.0 mg/L in 2019 of Monufia, Beheira and Beni Suef, respectively. Both chloropyrifos and alphacypermethrin were the lowest toxic to the three strains investigated.