The goal of this research was to establish a new genetic database of faba bean SSR primers and categories them according to their target genes and biological processes. Twenty-seven SSR PCR primers were synthesized to 12 Egyptian faba bean genotypes. Approximately 11 SSR provided one to two PCR bands, whereas other SSRs provided only one sharp band with polymorphic band size. There were 13 polymorphic primers. The polymorphism information content was 0.3, which implied moderate informativeness. The principle component analysis (PCA) was used to infer the genetic structure of the studied faba accessions using the data of applied SSR primers. The PCA analysis revealed 24.3% and 22.1% explanation of the genetic variation on both axes. The heatmap conducted using SSR data and clustered the faba beans into three groups, where Noubaria 1, Noubaria 2, Sakha 1 and Giza 3 were differentiated by their high genetic variation compared to other accessions.