This investigation was designed and implanted during two successive seasons 2019 and 2020 in the Experimental Farm of Horticulture Department, Faculty of Agriculture at Moshtohor, Benha University Qalyubeia Governorate, Egypt. Two grape varieties for export were chosen for this investigation (Crimson seedless and Mid Night Beauty) to study the effect of NPK mineral and bio- fertilization on vegetative growth of transplants at one-year-old. Treatments were the combination of eleven fertilization treatments (T1): Chemical fertilizer (NPK) at 6 g ammonium sulphate, 3g calcium superphosphate and 3 g potassium sulphate (N6g + P3g + K3g), (T2): Chemical fertilizer (NPK) at 9 g ammonium sulphate, 6g calcium superphosphate and 6 g potassium sulphate (N9g + P6g + K6g), (T3): N6g + P3g + K3g + 5g mixture of bio-fertilizers (Nitrobein, Phosphorene and Potassein), (T4): N6g + P3g + K3g + 10g mixture of bio-fertilizer, (T5): N6g + P3g + K3g + 15g mixture of bio-fertilizer, (T6): N9g + P6g + K6g + 5g mixture of bio-fertilizer, (T7): N9g + P6g + K6g + 10g mixture of bio-fertilizer, (T8): N9g + P6g + K6g + 15g mixture of bio-fertilizer, (T9): 5g mixture of bio-fertilizers, (T10): 10g mixture of bio-fertilizers and (T11): 15g mixture of bio-fertilizers/ transplants. The results indicated that,  Crimson seedless transplants resulted in an increase in length, leaf area, shoot fresh and dry weight whereas, Mid Night Beauty transplants resulted in an increase in stem diameter, number of lateral shoot, leaves dry weight, root fresh weight and root dry weight. Fertilization with T8 (N 9g +P 6g +K 6g +Bio 15g)  was superior in this respect where it was able to increase significantly all vegetative growth measurements as compared with the other different investigated fertilization during both seasons of study. On the other hand, treated transplants with T1 (Control) induced the lowest values in this concern in most cases.