This investigation was designed and implanted during two successive seasons 2019 and 2020 in the Experimental Farm of Horticulture Department, Faculty of Agriculture at Moshtohor, Benha University Qalyubeia Governorate, Egypt. Two grape cultivars for export were chosen for this investigation (Crimson Seedless and Mid Night Beauty) to study the effect of mineral NPK and bio- fertilization on leaf mineral content of transplants at one-year-old. Treatments were the combination of eleven fertilization treatments (T1) as control: N6g + P3g + K3g, (T2): N9g + P6g + K6g, (T3): N6g + P3g + K3g + 5g mixture of bio-fertilizers (Nitrobein, Phosphorene and Potassein), (T4): N6g + P3g + K3g + 10g mixture of bio-fertilizer, (T5): N6g + P3g + K3g + 15g mixture of bio-fertilizer, (T6): N9g + P6g + K6g + 5g mixture of bio-fertilizer, (T7): N9g + P6g + K6g + 10g mixture of bio-fertilizer, (T8): N9g + P6g + K6g + 15g mixture of bio-fertilizer, (T9): 5g mixture of bio-fertilizers, (T10): 10g mixture of bio-fertilizers and (T11): 15g mixture of bio-fertilizers/ transplants. The results indicated that, in most cases there were significant differences in leaf mineral content between the two tested cultivars "Crimson and Mid Night Beauty" Fertilization with T8 ( N 9g + P 6g + K 6g + Bio 15g)  was superior treatment in increasing leaf minerals content as compared with the other different investigated fertilization treatments included the control in most cases during both seasons.