The present study was aimed to investigate the effect of flock age, air pressure during incubation period and litter types on blood plasma constituents of broiler chicks. A total number of 600 one day old hatching chicks (ROSS 308) were taken randomly nearly a similar in live body weight were used in this study. Chicks were randomly divided into equal 12 groups in a 2×2×3 factorial arrangement experiment according to flock age (younger breeder flock 33 WKS and older breeder flock 43 WKS), air pressure (normal 100719 and high, 1011000 Pascal) and three litter types (wood shaving, plastic and sand). The results obtained showed that, broiler chicks produced from younger breeder flock recorded significantly (p < 0.001) the higher levels of plasma total protein (TP), albumin (A) and globulin (G). However, broiler chicks produced from older breeder flock found to be significantly decreased levels of plasma triglycerides, total cholesterol, low density lipoprotein, aspartate aminotransferase (AST), alanine aminotransferase (ALT) creatinine, uric acid and malondialdehyde (MDA).Broiler chicks produced from eggs incubated at high air pressure (HAP) showed significant(p < 0.001)  the higher levels of plasma TP, A , G and MDA. While, chicks produced from eggs incubated at low air pressure decreased significantly the levels of plasma triglycerides, total cholesterol, LDL, AST, ALT, creatinine, uric acid and significantly increased plasma levels of high density lipoprotein (HDL) and glutathione peroxidase (GPX). Blood plasma constituents significantly varied due to the effect of litter type, it was found that broiler chicks raised on sand litter type showed the higher levels of plasma  TP, A and G. While, the lower levels of plasma triglycerides, total cholesterol, LDL, AST, ALT, creatinine, uric acid, MDA and higher levels of HDL and GPX were found in broiler chicks raised on plastic (PL) litter type. According to the interaction between the studied factors, it could be concluded that the interaction between 43 WKS × HAP ×PL seemed to be adequate to achieve the favorable results of blood parameters.